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Mobilization of [Ca2+]i in the monkey hippocampal slices during transient hypoxia-hypoglycemia and KCl-induced deporalization was analyzed by microfluorometric imaging and anti-PIP2 immunohistochemistry. Hypoxia-hypoglycemia provoked the largest [Ca2+]i mobilization of CA-1 temperature-dependently whereas [Ca2+]i mobilization by KCl-induced depolarization occurred independent of the temperature in CA-2. Immunohistochemical analysis of the hippocampus after hypoxiahypoglycemia showed an increased PIP2 staining Cheap Oakleys Online preferentially in the perikarya of CA-1 Fake Oakleys Online neurons. These data suggest that release of Ca2+ from intracellular stores caused by PIP2 breakdown may induce elevated [Ca2+]i.
Electron-density determinations, utilizing the measurement of Compton-scatter intensities at selected scattering angles, are influenced by the presence of multiple scattering of photons. The fractions of single scatter and multiple scatter have been assessed in samples of varying diameters, using three different measuring geometries. For large scattering angles (θ = 90°), multiple-scatter fractions range from 0.51 to 0.82; for small scattering angles (θ = 20°), multiple-scatter fractions range from 0.09 to 0.17. Ratios of multiple-to-single scatter are given, and related to the measuring geometry and scattering angle. The implications for non-invasive in vivo densitometry are presented.
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